Journal
JOURNAL OF DENTAL RESEARCH
Volume 82, Issue 10, Pages 776-780Publisher
INT AMER ASSOC DENTAL RESEARCHI A D R/A A D R
DOI: 10.1177/154405910308201003
Keywords
Dmp1 expression; bone; tooth; development; knockout mice
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Funding
- NIDCR NIH HHS [DE13480, DE00455, DE13221] Funding Source: Medline
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Dentin Matrix Protein I (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMPI is not essential for early mouse skeletal or dental development.
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