Journal
INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH
Volume 15, Issue 5, Pages 369-372Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.ijir.3901040
Keywords
sildenafil; Viagra (TM); phosphodiesterase-5; PDE5 inhibitors; cGMP
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Funding
- NIDDK NIH HHS [DK40029, DK58277] Funding Source: Medline
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Sildenafil, the active ingredient in Viagra(TM), ahs been purified from commercially available tablets. The purification, using Sephadex G25 chromatography under conditions of low ionic strength, is simple and inexpensive. Sildenafil purified according to this protocol has been characterized with respect to its IC50 for PDE5, its ultraviolet absorption profile, and by collision-induced dissociation fingerprinting, positive ion nanospray, and MALDI mass spectrometry. Tritated sildenafil (6 Ci/mmol) was prepared commercially using the sildenafil purified by this protocol and was verified to retain the potency of unlabeled sildenafil. This protocol and similar procedures will allow investigators to easily isolate sufficient amounts of sildenafil or other PDE5 inhibitors for conducting biochemical and in vitro studies of drug action.
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