Journal
ARCHIVES OF MICROBIOLOGY
Volume 196, Issue 7, Pages 481-488Publisher
SPRINGER
DOI: 10.1007/s00203-014-0984-0
Keywords
Magnetosome; Magnetotactic bacteria; MamC; Magnetococus marinus
Categories
Funding
- Ministerio de Educacion, Cultura y Deporte (Spain) [CGL2007-63859, CGL2010-18274]
- U.S. National Science Foundation (NSF) [EAR-0920299, EAR-0525297, EAR-0745808]
- U.S. NSF [EAR-0920718]
- U.S. DOE [C02-07CH11358]
- Ramon y Cajal
- [SC-12-384]
- Directorate For Geosciences
- Division Of Earth Sciences [0745808] Funding Source: National Science Foundation
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Magnetotactic bacteria are a diverse group of prokaryotes that biomineralize intracellular magnetosomes, composed of magnetic (Fe3O4) crystals each enveloped by a lipid bilayer membrane that contains proteins not found in other parts of the cell. Although partial roles of some of these magnetosome proteins have been determined, the roles of most have not been completely elucidated, particularly in how they regulate the biomineralization process. While studies on the localization of these proteins have been focused solely on Magnetospirillum species, the goal of the present study was to determine, for the first time, the localization of the most abundant putative magnetosome membrane protein, MamC, in Magnetococcus marinus strain MC-1. MamC was expressed in Escherichia coli and purified. Monoclonal antibodies were produced against MamC and immunogold labeling TEM was used to localize MamC in thin sections of cells of M. marinus. Results show that MamC is located only in the magnetosome membrane of Mc. marinus. Based on our findings and the abundance of this protein, it seems likely that it is important in magnetosome biomineralization and might be used in controlling the characteristics of synthetic nanomagnetite.
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