Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 23, Issue 19, Pages 6769-6779Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.23.19.6769-6779.2003
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Funding
- NIGMS NIH HHS [R01 GM033523, R37 GM033523, F32GM20633-01, GM33523, F32 GM020633] Funding Source: Medline
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In a Xenopus egg replication system, the origin recognition complex (ORC) does not bind to CpG methylated DNA and DNA replication is inhibited. Insertion of low density CpG DNA of at least 1.2 kb into methylated plasmids rescues both replication and ORC binding. Using this pseudo-origin, we find that ORC binding is restricted to low-CpG-density DNA; however, MCM is loaded onto both weakly and highly methylated DNA and occupies at least similar to2 kb of DNA. Replication initiates coincident with MCM, and even the most distally bound MCM is associated with sites of replication initiation. These results suggest that in metazoans MCM is loaded onto and initiates replication over a large region distant from ORC.
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