Journal
ARCHIVES OF MICROBIOLOGY
Volume 195, Issue 10-11, Pages 749-757Publisher
SPRINGER
DOI: 10.1007/s00203-013-0927-1
Keywords
Optimization; Exopolysaccharides; Pleurotus eryngii; Antioxidant; Antitumour
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An optimal medium for exopolysaccharides (EPS) production was obtained through one-factor-at-a-time method and response surface methodology. Under optimal culture medium, the maximum EPS concentration in shake flask was 5.16 g/l. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography/multiangle laser light scattering, and the weight average molar masses (M (w)) of Fr-I and Fr-II were determined to be 4.098 x 10(4) and 1.114 x 10(4) g/mol, respectively. The molecular confirmation of Fr-I was revealed to be a rigid rod form in aqueous solution. Moreover, monosaccharide composition and characteristic groups were investigated by GC and Fourier transform infrared, respectively. Finally, pharmacology experiment in vitro indicated EPS Fr-II of Pleurotus eryngii exhibited higher antioxidant and antitumor abilities than Fr-I, which might be attributed to the different molecular weights and chemical compositions in the EPS fraction.
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