Journal
NMR IN BIOMEDICINE
Volume 16, Issue 6-7, Pages 400-412Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/nbm.840
Keywords
C-13 NMR spectroscopy; in vivo; brain; quantitation; LCModel; prior knowledge; isotopomers
Funding
- NCRR NIH HHS [P41 RR 08079] Funding Source: Medline
- NINDS NIH HHS [R01 NS 38672] Funding Source: Medline
Ask authors/readers for more resources
The LCModel method was adapted to analyze localized in vivo C-13 NMR spectra obtained from the rat brain in vivo at 9.4 T. Prior knowledge of chemical-shifts, J-coupling constants and J-evolution was included in the analysis. Up to 50 different isotopomer signals corresponding to 10 metabolites were quantified simultaneously in 400 mul volumes in the rat brain in vivo during infusion of [1,6- C-13(2)]glucose. The analysis remained accurate even at low signal-to-noise ratio of the order of 3:1. The relative distribution of isotopomers in glutamate, glutamine and aspartate determined in vivo in 22 min was in excellent agreement with that measured in brain extracts. Quantitation of time series of C-13 spectra yielded time courses of total C-13 label incorporation into up to 16 carbon positions, as well as time courses of individual isotopomer signals, with a temporal resolution as low as 5 min (dynamic isotopomer analysis). The possibility of measuring in vivo a wealth of information that was hitherto accessible only in extracts is likely to expand the scope of metabolic studies in the intact brain. Copyright (C) 2003 John Wiley Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available