Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 41, Issue 10, Pages 4865-4869Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.41.10.4865-4869.2003
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Funding
- NIAID NIH HHS [AI26170, R01 AI026170, R37 AI026170] Funding Source: Medline
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Luciferase reporter phages (LRPs) have proven to be efficient tools for drug susceptibility testing of Mycobacterium tuberculosis. Luminometric detection of LRP activity offers higher sensitivity and quantitative results, while a Polaroid film detection method offers a low-tech inexpensive alternative that is called the Bronx box. In this work we evaluated, improved, and compared the performance of the luminometer and the Bronx box formats for drug susceptibility testing with LRPs by using 51 clinical isolates of M. tuberculosis, with the agar proportion method (PM) serving as reference. The sensitivity in detecting resistance to isoniazid and rifampin, antibiotics that define multidrug resistance (MDR), was 100% for both methods. The turnaround time for results was reduced from 3 weeks for PM to 54 or 94 h for luminometry or the Bronx box, respectively. These results support the utility of LRPs as a screening test for the surveillance of MDR tuberculosis.
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