Journal
ARCHIVES OF MICROBIOLOGY
Volume 193, Issue 9, Pages 677-685Publisher
SPRINGER
DOI: 10.1007/s00203-011-0710-0
Keywords
Bacillus subtilis; SigI; Cell wall hydrolase; LytE; Heat stress
Categories
Funding
- National Science Council [NSC 96-2311-B-010-002]
- Ministry of Education of the Republic of China
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The Bacillus subtilis cell wall hydrolase LytE is involved in cell wall turnover and cell separation during vegetative growth. lytE transcription is known to be driven by a YycF-activated SigA-dependent promoter. The cell wall regulator SigI is an alternative sigma factor that has been shown to be heat stress-inducible and to be essential for survival of B. subtilis at high temperature. However, none of the previously identified target genes of SigI contribute to heat resistance. We now demonstrate that lytE expression is heat-inducible and that heat induction of lytE expression is strongly dependent on SigI. We have also found that the lytE mutant shows the same growth defect at high temperature as the sigI mutant. Introducing an extra copy of lytE into the sigI mutant could rescue its growth defect. Our data strongly suggest that SigI-dependent lytE expression under heat stress is important for heat survival of B. subtilis.
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