Journal
ARCHIVES OF MICROBIOLOGY
Volume 191, Issue 12, Pages 885-894Publisher
SPRINGER
DOI: 10.1007/s00203-009-0517-4
Keywords
Burkholderia kururiensis subsp kururiensis; Burkholderia kururiensis subsp thiooxydans; Mixotrophic growth; Reduced sulfur compounds oxidation; Thiosulfate-oxidizing bacteria
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Funding
- Rural Development Administration (RDA), Republic of Korea
- National Academy of Agricultural Science, RDA, Republic of Korea
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A thiosulfate-oxidizing facultative chemolithoautotrophic Burkholderia sp. strain ATSB13(T) was previously isolated from rhizosphere soil of tobacco plant. Strain ATSB13(T) was aerobic, Gram-staining-negative, rod shaped and motile by means of sub-terminal flagellum. Strain ATSB13(T) exhibited mixotrophic growth in a medium containing thiosulfate plus acetate. A phylogenetic study based on 16S rRNA gene sequence analysis indicated that strain ATSB13(T) was most closely related to Burkholderia kururiensis KP23(T) (98.7%), Burkholderia tuberum STM678(T) (96.5%) and Burkholderia phymatum STM815(T) (96.4%). Chemotaxonomic data [G+C 64.0 mol%, major fatty acids, C-18:1 omega 7c (28.22%), C-16:1 omega 7c/15 iso 2OH (15.15%), and C-16:0 (14.91%) and Q-8 as predominant respiratory ubiquinone] supported the affiliation of the strain ATSB13(T) within the genus Burkholderia. Though the strain ATSB13(T) shared high 16S rRNA gene sequence similarity with the type strain of B. kururiensis but considerably distant from the latter in terms of several phenotypic and chemotaxonomic characteristics. DNA-DNA hybridization between strain ATSB13(T) and B. kururiensis KP23(T) was 100%, and hence, it is inferred that strain ATSB13(T) is a member of B. kururiensis. On the basis of data obtained from this study, we propose that B. kururiensis be subdivided into B. kururiensis subsp. kururiensis subsp. nov. (type strain KP23(T) = JCM 10599(T) = DSM 13646(T)) and B. kururiensis subsp. thiooxydans subsp. nov. (type strain ATSB13(T) = KACC 12758(T)).
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