Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 96, Issue 4, Pages 317-323Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(03)90130-7
Keywords
PCR bias; chimera; heteroduplex; microbial community analysis; 16S rRNA gene
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Polymerase chain reaction (PCR) is often used for the amplification of a mixture of homologous genes. PCR bias and artifact formation can occur in multitemplate PCR, and provide incorrect information on the abundance and diversity of genes. PCR bias and artifact formation occur at a higher rate during the last few cycles of the reaction, and therefore can be avoided by stopping the PCR earlier.
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