Journal
JOURNAL OF BIOMATERIALS APPLICATIONS
Volume 18, Issue 2, Pages 137-148Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/088532803038452
Keywords
monoclonal antibodies; immobilization; fabrics; cell culture
Ask authors/readers for more resources
Mouse hybridoma cells (O1468) producing a monoclonal antibody against the 1465 antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available