Journal
FERTILITY AND STERILITY
Volume 80, Issue 4, Pages 921-929Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(03)01125-7
Keywords
Ureaplasma parvum; Ureaplasma urealyticum; semen; washed semen; assisted reproductive technology; adherence; spermatozoa motility
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Objective: To investigate the prevalence of ureaplasmas in semen and washed semen and to explore their effect on semen andrology variables. Design: Prospective study. Setting: In vitro fertilization (IVF) unit of a private hospital. Patient(s): Three hundred forty-three men participating in an assisted reproductive technology (ART) treatment cycle. Main Outcome Measure(s): The prevalence of ureaplasmas in semen and washed semen tested by culture, polymerase chain reaction assays, and indirect immunofluorescent antibody assays. Result(s): Ureaplasmas were detected in 73 of 343 (22%) semen samples and 29 of 343 (8.5%) washed semen samples. Ureaplasmas adherent to the surface of spermatozoa were demonstrated by indirect immunofluorescent antibody testing. Ureplasma parvum serovar 6 (36.6%) and U. urealyticum (30%) were the most prevalent isolates in washed semen. A comparison of the semen andrology variables of washed semen ureaplasma positive and negative groups demonstrated a lower proportion of nonmotile sperm in men ureaplasma positive for washed semen. Conclusion(s): Ureaplasmas are not always removed from semen by a standard ART washing procedure and can remain adherent to the surface of spermatozoa. (C)2003 by American Society for Reproductive Medicine.
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