Journal
FEMS YEAST RESEARCH
Volume 4, Issue 1, Pages 69-78Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1567-1356(03)00141-7
Keywords
xylose isomerase; hemicellulose; fermentation; pentose; yeast; bioethanol
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Evidence is presented that xylose metabolism in the anaerobic cellulolytic fungus Piromyces sp. E2 proceeds via a xylose isomerase rather than via the xylose reductase/xylitol-dehydrogenase pathway found in xylose-metabolising yeasts. The XylA gene encoding the Piromyces xylose isomerase was functionally expressed in Saccharomyces cerevisiae. Heterologous isomerase activities in cell extracts, assayed at 30degreesC, were 0.3-1.1 mumol min(-1) (mg protein)(-1), with a K-m for xylose of 20 mM. The engineered S. cerevisiae strain grew very slowly on xylose. It co-consumed xylose in aerobic and anaerobic glucose-limited chemostat cultures at rates of 0.33 and 0.73 mmol (g biomass)(-1) h(-1), respectively. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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