Journal
NUCLEIC ACIDS RESEARCH
Volume 31, Issue 19, Pages 5732-5740Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg758
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Funding
- NIAID NIH HHS [AI 47673, R01 AI047673] Funding Source: Medline
- NIGMS NIH HHS [GM 58447] Funding Source: Medline
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Eilatin-containing ruthenium complexes bind to a broad range of different nucleic acids including: calf thymus (CT) DNA, tRNA(Phe), polymeric RNAs and DNAs, and viral RNAs including the HIV-1 RRE and TAR. The nucleic acid specificity of Lambda- and Delta-[Ru(bpy)(2)eilatin](2+) have been compared to that of the 'free' eilatin ligand, and to the classic intercalating agent ethidium bromide. Interestingly, all four compounds appear to bind to nucleic acids by intercalation, but the trends in nucleic acid binding specificity are highly diverse. Unlike ethidium bromide, both eilatin and the eilatin-containing coordination complexes bind to certain single-stranded RNAs with high affinity (K(d)less than or equal to1 muM). Eilatin itself is selective for electron-poor polymeric purines, while the eilatin-coordination complexes exhibit preference for the polypyrimidine r(U). These results show how the binding specificity of an intercalating ligand can change upon its incorporation into an octahedral metal complex.
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