Journal
METHODS
Volume 31, Issue 2, Pages 160-171Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1046-2023(03)00126-9
Keywords
T cells; MFIC-tetramer complexes; CTL; immune monitoring
Funding
- NIAID NIH HHS [R01 AI 14870, P01 AI43664] Funding Source: Medline
- NIAMS NIH HHS [N01 AR9 2239] Funding Source: Medline
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Peptide-major histocompatibility complexes (MHC) class I tetrameric complexes (tetramers) are proving invaluable as fluorescent reagents for enumeration, characterization, and isolation of peptide-specific T cells and have afforded many advantages over previous techniques, particularly the ability to directly quantify and phenotype antigen-specific T cells with minimal in vitro manipulation. Recently, various technical peculiarities of tetramers have led to modifications within this important immunoassay. The current manuscript details potential pitfalls and provides guidance for interpretation of results. (C) 2003 Elsevier Science (USA). All rights reserved.
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