Journal
JOURNAL OF VIROLOGY
Volume 77, Issue 20, Pages 11290-11295Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.77.20.11290-11295.2003
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We assayed the infectivity of naked foot-and-mouth disease virus (FMDV) RNA by direct inoculation of suckling mice. Our results demonstrate that transcripts generated from full-length cDNA clones were infectious, as was virion-extracted RNA. Interestingly, infectious virus could be recovered from a mutant transcript encoding amino acid substitution L-147-->P in capsid protein VP1, known to be noninfectious for BHK-21 cells. The model described here provides a useful tool for virulence studies in vivo, bypassing possible selection of variants during viral replication in cell culture.
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