Journal
EXTREMOPHILES
Volume 7, Issue 5, Pages 409-413Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s00792-003-0336-5
Keywords
fluorescence in situ hybridization; halophiles; in situ physiology; microautoradiography; Salinibacter; square archaea; uncultured microorganisms
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The combination of fluorescence in situ hybridization and microautoradiography (FISH-MAR approach) was applied to brine samples of a solar saltern crystallizer pond from Mallorca (Spain) where the simultaneous occurrence of Salinibacter spp. and the conspicuous square Arehaea had been detected. Radioactively labeled bicarbonate, acetate, glycerol, and an amino acid mixture were tested as substrates for the microbial populations inhabiting such brines. The results indicated that hitherto uncultured 'square Archaea' do actively incorporate amino acids and acetate. However, Salinibacter spp. only showed am I no acid incorporation in pure culture, but no evidence of such activity in their natural environment could be demonstrated. No glycerol incorporation was observed for any component of the microbial community.
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