Journal
BIOPHYSICAL CHEMISTRY
Volume 106, Issue 1, Pages 23-29Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0301-4622(03)00138-8
Keywords
transcriptional activity; DNA condensation; higher-order structure of DNA; spermine; poly(ethylene glycol); fluorescence microscopy
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We found that the transcriptional activity of large DNAs (40 kbp) can be completely inhibited by adding condensing agents, spermine and poly(ethylene glycol), whereas under the same conditions short fragments (140 bp) still show active transcription. Fluorescence microscopic observations of large DNAs revealed clear correlation between the higher-order structure of templates and their transcriptional activity. The steep decrease in transcriptional activity leading to complete inhibition, or on/off switching, is interpreted in terms of conformational transition of the ensemble of DNA molecules. (C) 2003 Elsevier Science B.V. All rights reserved.
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