Journal
JOURNAL OF WILDLIFE DISEASES
Volume 39, Issue 4, Pages 881-886Publisher
WILDLIFE DISEASE ASSN, INC
DOI: 10.7589/0090-3558-39.4.881
Keywords
Ehrlichia chaffeensis; experimental infection; Odocoileus virginianus; white-tailed deer
Categories
Funding
- PHS HHS [R15A147413-01] Funding Source: Medline
Ask authors/readers for more resources
The infection dynamics of the tick-transmitted organism Ehrlichia chaffeensis were investigated in white-tailed deer (Odocoileus virginianus) using different routes of inoculation. Six deer were each inoculated with 5.4x10(6) DH82 cells infected with E. chaffeensis (Arkansas strain) by three different routes: intravenous (n=2), subcutaneous (n=2), and intradermal (n=2). Two control deer were inoculated with uninfected cells. Infections were monitored for 54 days and were continued in one deer from each E. chaffeensis inoculated group for an additional 31 days. All deer inoculated with E. chaffeensis seroconverted ( greater than or equal to1: 64) and became 16S rDNA polymerase chain reaction and/or cell culture positive by post-inoculation day 15. There was no apparent difference in susceptibility to infection between deer inoculated by different routes for the first 50 days based on detection of E. chaffeensis infection by PCR assay of blood or culture isolation. These results demonstrate infection of deer by intradermal and subcutaneous routes for the first time.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available