Journal
APPLIED PHYSICS B-LASERS AND OPTICS
Volume 77, Issue 4, Pages 377-380Publisher
SPRINGER-VERLAG
DOI: 10.1007/s00340-003-1280-x
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We demonstrate sub-diffraction lateral resolution of 28 +/- 2 nm in far-field fluorescence microscopy through stimulated emission depletion effected by an amplified laser diode. Measurement of the optical transfer function in the focal plane reveals a 6-fold enlargement of the spatial bandwith over the diffraction limit. The resolution is established by imaging individual fluorescent molecules on a surface. Corresponding to 1/25 of the responsible wavelength, the attained resolution represents a new benchmark in far-field microscopy and underscores the viability of fluorescence nanoscopy with visible light, conventional optics and compact laser systems.
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