Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 795, Issue 2, Pages 179-186Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1570-0232(03)00565-8
Keywords
phosphodiesterase inhibitor; DA-8159
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A sensitive high-performance liquid chromatographic (HPLC) method with ultraviolet absorption detection (292 nm) was developed and validated for the determination of the new phosphodiesterase V inhibitor, DA-8159 (DA), in human plasma and urine. A single step liquid-liquid extraction procedure using ethyl ether was performed to recover DA and the internal standard (sildenafil citrate) from 1.0 ml of biological matrices combined with 200 mul of 0.1 M sodium carbonate buffer. A Capcell Pak C(18)UG120 column (150 mm x 4.6 mm I.D., 5 mum) was used as a stationary phase and the mobile phase consisted of 30% acetonitrile and 70% 20 mM potassium phosphate buffer (pH 4.5) at a flow rate of 1.0 ml/min. The lower limit for quantification was 5 ng/ml for plasma and 10 ng/ml for urine samples. Within- and between-run accuracy and precision were less than or equal to 15 and less than or equal to 10%, respectively, in both plasma and urine samples. The recovery of DA from human plasma and urine was greater than 70%. Separate stability studies showed that DA is stable under the conditions of analysis. This validated assay was used for the pharmacokinetic analysis of DA during a phase I, rising dose study. (C) 2003 Elsevier B.V. All rights reserved.
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