Journal
ENGINEERING IN LIFE SCIENCES
Volume 3, Issue 10, Pages 401-405Publisher
WILEY-BLACKWELL
DOI: 10.1002/elsc.200301831
Keywords
Polysaccharides; Enzymatic hydrolysis; Microwaves
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Brewer's spent grains (BSG), the voluminous residue after mashing, contains on dry weight basis about 40-50 % polysaccharides. For the recovery of soluble carbohydrates from BSG different physical, thermal and enzymatic treatments were used to solubilize the polysaccharides in BSG. Heating by microwave radiation to 160 degrees C in the presence of 0.1 M HCl released 35 % of the material in the form of reducing sugar, indicating that about 80 % of the polysaccharides were hydrolyzed. Nevertheless, 0.1 M acetic acid will even solubilize 30 % of the material as oligosaccharides on a prolonged incubation when pretreating the spent grains by extrusion cooking. A combination of the enzymes Ceremix Plus MG and CelluPract AL 70 achieved an almost 25 % release of saccharides after 4 hrs of incubation at 50 degrees C. A combination of extrusion cooking and enzymatic hydrolysis seems to be a very promising procedure.
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