Journal
ANALYTICA CHIMICA ACTA
Volume 494, Issue 1-2, Pages 187-197Publisher
ELSEVIER
DOI: 10.1016/S0003-2670(03)00919-X
Keywords
self-assembled monolayers; tyrosinase; flowing systems; gold disk electrode
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The performance of a tyrosinase (Tyr) biosensor, constructed by immobilisation of the enzyme by cross-linking atop a 3-mercaptopropionic acid (MPA) self-assembled monolayer (SAM) on a An disk electrode, is reported for the amperometric detection under flow-injection (FI) conditions of several phenolic compounds (phenol, catechol, m-cresol, p-cresol, 4-chloro-3-methylphenol, 3-chlorophenol, 4-chlorophenol, 2,4-dimethylphenol, 3,4-dimethylphenol, and 2-aminophenol). Experimental variables such as the detection potential (-100 mV versus Ag\AgCl\KCl 3 M), flow rate (1.02 ml min(-1)), injection volume (350 RI), and pH of the carrier solution (0.05 M phosphate buffer of pH 7.0) were optimised. Under these conditions, the Tyr biosensor exhibited a good reproducibility of the FI measurements, with no need to apply a cleaning or pre-treatment procedure. The useful lifetime of one single biosensor was 5 days. The kinetic parameters of the Tyr reaction were calculated for the 10 phenolic compounds. The analytical performance of the Tyr-MPA-Au electrode under flow through conditions was compared with that of other Tyr electrochemical biosensors reported in the literature. The usefulness of the biosensor for the analysis of real samples was proved by performing the estimation of the content of phenolic compounds in waste waters from a refinery at three different stages in the waste purification process. (C) 2003 Published by Elsevier B.V.
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