Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 21, Pages 12396-12401Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1635160100
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Funding
- NCI NIH HHS [R01 CA085786, CA 82396, CA 85786, R01 CA082396, CA 87661] Funding Source: Medline
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Human cytomegalovirus has a complex double-stranded DNA genome of approximate to240,000 bp that contains approximate to150 ORFs likely to encode proteins, most of whose functions are not well understood. We have used an infectious bacterial artificial chromosome to introduce 413 defined insertion and substitution mutations into the human cytomegalovirus AD169 genome by random and site-directed transposon mutagenesis. Mutations were produced in all unique ORFs with a high probability of encoding proteins for which mutants have not been previously documented and in many previously characterized ORB. The growth of selected mutants was assayed in cultured human fibroblasts, and we now recognize 41 essential, 88 nonessential, and 27 augmenting ORI's. Most essential and augmenting genes are located in the central region, and nonessential genes generally cluster near the ends of the viral genome.
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