Journal
GENES & DEVELOPMENT
Volume 17, Issue 20, Pages 2502-2507Publisher
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gad.1133003
Keywords
EBR1; ADAMTS; TSP-1; hyalin; sea urchin; species specificity
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Species-specific adhesion of sperm to the egg during sea urchin fertilization involves the interaction of the sperm adhesive protein, bindin, and a complementary receptor on the egg surface, and serves to restrict the gene pool to individuals of the same species. We used PCR representation difference analysis to clone the species-specific egg receptor for bindin, EBR1, from Strongylocentrotus franciscanus (Sf) and S. purpuratus (Sp). Sf-EBR1 contains a novel ADAMTS-like N-terminal domain followed by similar to19 tandem EBR repeats consisting of alternating CUB and thrombospondin type 1 (TSP-1) domains where the last 10 EBR repeats are species-specific and highly conserved. Recombinant protein corresponding to the species-specific EBR repeat displays species-specific sperm adhesion and bindin-binding activity. The Sp-EBR1 ortholog has the same ADAMTS (a disintegrin and metalloprotease with thrombospondin type-1 modules) core region followed by eight and one-half tandem egg bindin receptor (EBR) repeats that share 88% identity with the Sf-EBR1 repeats, but has an entirely different species-specific domain consisting of hyalin-like (HYR) repeats. Thus, the species-specific domains of egg bindin receptor 1 (EBR1) from both species function as the egg surface receptor to mediate species-specific sperm adhesion.
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