Journal
MACROMOLECULAR BIOSCIENCE
Volume 3, Issue 10, Pages 531-534Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200300024
Keywords
biopolymers; chitosan; conductometric titration; degree of acetylation; UV-vis spectroscopy
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A simple and rapid method for determining the degree of N-acetylation (DA) of chitosan by ultraviolet spectrophotometry using double beam equipment is proposed. D-Glucosamine (GlcN) hydrochloride and N-acetylglucosamine (GlcNAc) were used as model substances, and chitosan solutions in different solvents were studied. The best results were obtained from 0.1 m HCl solutions, with high reproducibility and in good agreement with those given by conductometric titration, FT-IR, H-1 NMR and elemental analysis.
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