Journal
SCIENCE
Volume 302, Issue 5646, Pages 842-846Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1088305
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Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of similar to30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
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