Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1017, Issue 1-2, Pages 187-193Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2003.07.003
Keywords
tobacco; cigarette smoke; alkaloids; nicotine; nornicotine; myosmine; nicotyrine; anabasine; anatabine; 2,3-dipyridyl
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A novel fast megabore capillary gas chromatographic (MCGC) method for analysis of 7 nicotine related alkaloids in tobacco and cigarette smoke, including nicotine, nornicotine, myosmine, nicotyrine, anabasine, anatabine and 2,3-dipyridyl, was developed. The use of megabore capillary column GC methodology, equipped with flame ionization detector (FID), provided rapid, unambiguous nicotine related alkaloids analysis. One gram flue-cured tobacco (or Cambridge filter pad), 20 ml ether, and 5 ml 10% sodium hydroxide solution, added with n-heptadecane as the internal standard, were placed in a flask, and the flask was capped and placed in an ultrasonic bath for 15 min. A 1 mul volume was analyzed by capillary GC operating in split-injection mode on a megabore Simplicity(TM)-5 column. This simple procedure was compared with the previously reported packed column GC method and the Griffith still-colorimetric method. The application of the method for analysis of various flue-cured tobaccos and cigarette smoke was discussed. (C) 2003 Elsevier B.V. All rights reserved.
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