Journal
BIOCHEMISTRY-MOSCOW
Volume 68, Issue 11, Pages 1261-1266Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1023/B:BIRY.0000009142.88974.85
Keywords
immobilized subtilisin; poly(vinyl alcohol) cryogel; complex of subtilisin with poly(acrylic acid); organic solvents
Categories
Ask authors/readers for more resources
The activity and stability of native subtilisin 72, its complex with poly(acrylic acid), and subtilisin covalently attached to poly(vinyl alcohol) cryogel were studied in aqueous and organic media by hydrolysis of specific chromogenic peptide substrates. Kinetic parameters of the hydrolysis of Glp-Ala-Ala-Leu-pNA by native subtilisin and its complex with poly(acrylic acid) were determined. Based on the comparative study of stability of native and modified subtilisins in media of various compositions, it was established that covalent immobilization of subtilisin on poly(vinyl alcohol) cryogel is the most effective approach to improve enzyme stability in water as well as in mixtures with low water content.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available