Specific binding of vanadyl ion (VO2+) with thiolate of the cysteine-34 residue in serum albumin, demonstrated by CD spectroscopy and kinetic property

Binding feature of the vanadyl ion (VO2+) with bovine serum albumin (BSA) was studied with CD spectrometry based on comparisons with that of Cu2+. The strong positive CD band at 590 nm in the VO2+-albumin system was assigned to the binding of VO2+ and thiolate of Cys-34 in BSA.