Journal
JOURNAL OF CELL BIOLOGY
Volume 163, Issue 3, Pages 535-545Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200306001
Keywords
adhesion; cytoskeleton; endocytosis; cadherin; catenin
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Funding
- NIAMS NIH HHS [R01 AR048266, 1R01AR048266, P30AR042687, P30 AR042687] Funding Source: Medline
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T =he mechanisms by which catenins regulate cadherin function are not fully understood, and the precise function of p120 catenin (p120ctn) has remained particularly elusive. In microvascular endothelial cells, p120ctn colocalized extensively with cell surface VE-cadherin, but failed to colocalize with VE-cadherin that had entered intracellular degradative compartments. To test the possibility that p120ctn binding to VE-cadherin regulates VE-cadherin internalization, a series of approaches were undertaken to manipulate p120ctn availability to endogenous VE-cadherin. Expression of VE-cadherin mutants that competed for p120ctn binding triggered the degradation of endogenous VE-cadherin. Similarly, reducing levels of p120ctn using siRNA caused a dramatic and dose-related reduction in cellular levels of VE-cadherin. In contrast, overexpression of p120ctn increased VE-cadherin cell surface levels and inhibited entry of cell surface VE-cadherin into degradative compartments. These results demonstrate that cellular levels of p120ctn function as a set point mechanism that regulates cadherin expression levels, and that a major function of p120ctn is to control cadherin internalization and degradation.
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