Journal
ANALYTICA CHIMICA ACTA
Volume 497, Issue 1-2, Pages 93-99Publisher
ELSEVIER
DOI: 10.1016/j.aca.2003.08.050
Keywords
dopamine; ethylene diamine; fluorimetry; injection; urine
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A sensitive and selective method for the determination of dopamine is described. Dopamine was oxidized by mercury(II) nitrate and the oxidation product was condensed with ethylene diamine to form a quinoxaline derivative which was strongly fluorescent. The measurement was carried out at 447 nm with excitation at 393 nm. Effects of pH, oxidants, and foreign ions on the determination of dopamine were examined. A linear relationship was obtained between the relative fluorescence intensity (RFI) and the concentration of dopamine in the range of 0.02 to 0.06 mug ml(-1). The linear regression equation of the calibration graph is C = 0.001347F - 0.02564 (C is concentration of dopamine (mug ml(-1)) and F is relative fluorescence intensity in the equation), with a correlation coefficient of 0.9991 and a relative standard deviation of 4.4%. Dopamine was separated from adrenaline and noradrenaline in urine by thin layer chromatography. The detection limit is 18 ng ml(-1), and the recovery is from 95.0 to 106.6%. This method can be used for the determination of dopamine in injection and urine samples. (C) 2003 Elsevier B.V. All rights reserved.
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