Journal
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
Volume 13, Issue 22, Pages 4129-4132Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmcl.2003.07.032
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Funding
- NCRR NIH HHS [RR 10485] Funding Source: Medline
- NIGMS NIH HHS [GM 32634, GM 58481] Funding Source: Medline
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Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of (H2O)-O-18 and O-18(2), and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water. (C) 2003 Elsevier Ltd. All rights reserved.
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