Journal
FEBS LETTERS
Volume 554, Issue 3, Pages 394-398Publisher
WILEY
DOI: 10.1016/S0014-5793(03)01197-9
Keywords
green fluorescent protein; proteomics; functional analysis; high-content screening microscopy; membrane traffic; Golgi complex
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A modular microscope-based screening platform, with applications in large-scale analysis of protein function in intact cells is described. It includes automated sample preparation, image acquisition, data management and analysis, and the genome-wide automated retrieval of bioinformatic information. The modular nature of the system ensures that it is rapidly adaptable to new biological questions or sets of proteins. Two automated functional assays addressing protein secretion and the integrity of the Golgi complex were developed and tested. This shows the potential of the system in large-scale, cell-based functional proteomic projects. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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