Second-harmonic generation as a probe of conformational change in molecules

Optical second-harmonic generation has been used to detect a ligand-induced conformational change in a protein. The protein was made second-harmonic active by labeling it with a second-harmonic active dye. The conformational change was detected in a monolayer of the protein at the air-water interface. (C) 2003 Elsevier B.V. All rights reserved.