Effect of protein-protein interactions on protein aggregation kinetics

The mutual diffusion coefficient D-m of dilute lysozyme solutions is measured by the dynamic light scattering technique. The relation between the osmotic second virial coefficient B-22 and the protein mutual diffusion coefficient D-m of dilute solutions is examined experimentally and theoretically. The role of B-22 in relation with D-m in protein aggregation kinetics was analyzed based on the effect of the average protein pairwise interactions on the protein aggregation kinetics. The empirical crystallization window'' proposed by George and Wilson [Acta Crystallogr., Sect. D: Biol. Crystallogr. 50, 361 (1994)] can be interpreted from the point view of crystallization kink kinetics. (C) 2003 American Institute of Physics.