Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 24, Pages 14309-14314Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1835673100
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Funding
- NCI NIH HHS [R01 CA074730, CA74730] Funding Source: Medline
- NIAID NIH HHS [AI45019, T32 AI007163, T32 AI07163, R01 AI045019] Funding Source: Medline
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Macrophages (Mphi) are activated by IFNgamma and are important cellular targets for infection by human and murine cytomegalovirus (MCMV), making it advantageous for CMVs to block IFNgamma-induced Mphi differentiation. We found that MCMV infection inhibited IFNgamma regulation of many genes in Mphi. MCMV infection blocked IFNgamma responses at the level of transcription without blocking Janus kinase/signal transducer and activator of transcription pathway activation and targeted IFN response factor 1- and class 11 transactivator-dependent and independent promoters. MCMV did not alter basal transcription from IFNgamma-responsive promoters and left the majority of cellular transcripts unchanged even after 48 h of infection. The effects of MCMV infection were specific to chromosomal rather than transiently transfected promoters. Characterization of the IFNgamma-responsive chromosomal class 11 transactivator promoter revealed that MCMV infection blocked IFNgamma-induced promoter assembly, allowing the virus to transcriptionally paralyze infected Mphi responses while allowing basal transcription to proceed.
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