Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 24, Pages 13887-13891Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2036346100
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Funding
- NIGMS NIH HHS [R01 GM036659, R37 GM036659, GM36659] Funding Source: Medline
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The multiprotein Mediator complex has been shown to interact with gene-specific regulatory proteins and RNA polymerase 11 in vitro. Here, we use chromatin immunoprecipitation to analyze the recruitment of Mediator to GAL genes of yeast in vivo. We find that Mediator associates exclusively with transcriptionally active and not inactive GAL genes. This association maps to the upstream activating sequence, rather than the core promoter, and is independent of RNA polymerase 11, general transcription factors, and core promoter sequences. These findings support the idea of Mediator as a primary conduit of regulatory information from enhancers to promoters in eukaryotic cells.
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