Journal
PROCESS BIOCHEMISTRY
Volume 39, Issue 3, Pages 387-391Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0032-9592(03)00127-4
Keywords
PHAs; Ralstonia eutropha; mixed organic acids; continuous feeding
Ask authors/readers for more resources
Feeding strategies of feeding mixed organic acids as carbon sources for the biosynthesis of polyhydroxyalkanoates (PHAs) by Ralstonia eutropha were investigated. A feeding strategy was proposed according to the singular optimization control theory after comparing several feeding strategies of mixed acids for PHAs formation which referred to that the culture was absent of carbon sources firstly, and mixed acids was fed throughout the procedure at varying rates, additionally, to keep the cell more active, nitrogen source (sulfate ammonia) was also fed from about the 12th hour of the fermentation. In contrast with the batch culture in which the maximum dry cell weight and PHAs concentration were only 3.89 and 1.45 g/l, the corresponding values of the continuous feeding fed-batch culture were increased to 14.35 and 6.89 g/l, respectively. (C) 2003 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available