Journal
BIOCATALYSIS AND BIOTRANSFORMATION
Volume 21, Issue 6, Pages 325-331Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10242420310001630191
Keywords
dextransucrase; immobilization; LentiKats; alginate; polyvinyl alcohol hydrogels; encapsulation; acceptor reaction; functional foods
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Insoluble (cell-bound) dextransucrase from Leuconostoc mesenteroides B-1299 was encapsulated in highly elastic and stable hydrogels formed by polyvinyl alcohol. The gelation was carried out by controlled partial drying at room temperature, resulting in lens-shaped particles, called LentiKats. A similar recovery of activity (approximately 55%) was achieved when compared with entrapment in calcium alginate gels. Under reaction conditions, the protein leakage in LentiKats was reduced from 18% to 4% by pre-treatment of the dextransucrase with glutaraldehyde. The immobilized dextransucrases were tested in the acceptor reaction with methyl alpha-D-glucopyranoside. The conversion to oligosaccharides using Lentikat-dextransucrase was higher than that obtained for alginate-dextransucrase, probably due to the reduction of diffusional limitations derived from its lenticular shape. In addition, a shift of selectivity towards the synthesis of oligosaccharides containing alpha(1-->2) bonds was observed for the Lentikat-biocatalysts. These non-digestible compounds are supposed to be specifically fermented by beneficial species of the human microflora (prebiotic effect ) . The Lentikat-entrapped dextransucrase can be efficiently reused in this process at least for five cycles of 24 h.
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