Journal
JOURNAL OF DENTAL RESEARCH
Volume 82, Issue 12, Pages 951-956Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/154405910308201203
Keywords
TMJ; osteochondral; tissue engineering; cartilage; bone
Categories
Funding
- NIDCR NIH HHS [DE13964, DE15391] Funding Source: Medline
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The temporomandibular joint is susceptible to diseases and trauma that may ultimately lead to structural degeneration. Current approaches for replacing degenerated mandibular condyles suffer from deficiencies such as donor site morbidity, immunorejection, implant wear and tear, and pathogen transmission. The hypothesis of this study was that a human-shaped mandibular condyle can be tissue-engineered from rat mesenchymal stem cells (MSCs) encapsulated in a biocompatible polymer. Rat bone marrow MSCs were isolated and induced to differentiate into chondrogenic and osteogenic cells in vitro, and encapsulated in poly(ethylene glycol)-based hydrogel in two stratified layers molded into the shape of a cadaver human mandibular condyle. Eight weeks following in vivo implantation of the bilayered osteochondral constructs in the dorsum of immunodeficient mice, mandibular condyles formed de novo. Microscopic evaluation of the tissue-engineered mandibular condyle demonstrated two stratified layers of histogenesis of cartila-inous and osseous phenotypes. The current approach is being refined for ultimate therapeutic applications.
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