Journal
PLANT AND CELL PHYSIOLOGY
Volume 44, Issue 12, Pages 1412-1416Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcg161
Keywords
Cryptomeria japonica; phytosulfokine; regeneration; somatic embryogenesis; Sugi; zygotic embryo
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Phytosulfokine (PSK), which has been identified as a plant growth factor, had a dramatic stimulatory effect on the formation of somatic embryos of sugi (Cryptomeria japonica) in the presence of polyethylene glycol. The resultant somatic embryos germinated with synchronous sprouting of cotyledons, hypocotyls and roots, and most of the seedlings grew normally. A cDNA clone for the precursor to the PSK peptide of C. japonica was identified in an expressed sequence tags database. Our results support the existence of a PSK signaling pathway in C. japonica.
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