Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 33, Issue 7, Pages 987-990Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(03)00253-9
Keywords
adventitious hairy root; bacterial elicitors; Scopolia parviflora; tropane alkaloids; H6H; PMT
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In an attempt to increase productivity, the effect of elicitation on tropane alkaloids (TA) biosynthesis was studied in adventitious hairy root cultures of Scopolia parviflora. Two Gram-positive strains and one Gram-negative strain of bacteria were used as biotic elicitors. The raw bacterial elicitors affected the tropane alkaloid profile by increasing the scopolamine concentration, while the autoclaved bacterial elicitors produced similar effects on the control. The conversion ratio of hyoscyamine to scopolamine was increased following elicitation using raw bacterial elicitors. The bacterial elicitor inhibited the expression of H6H (hyoscyamine 6beta-hydoxylase) whereas the expression of PMT (putrescine N-methyltransferase) was raised by elicitation. These results have important implications for the large-scale production of tropane alkaloids. (C) 2003 Elsevier Inc. All rights reserved.
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