Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 33, Issue 5, Pages 1135-1142Publisher
ELSEVIER
DOI: 10.1016/S0731-7085(03)00426-6
Keywords
high performance liquid chromatography; direct injection; benzodiazepines; Hisep column; plasma
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A direct plasma injection HPLC method has been developed for the determination of selected benzodiazepines (nitrazepam, clobazam, oxazepam, lorazepam). The method uses an analytical hydrophobic shielded phase (Hisep) column equipped with a Hisep guard column, are easy to perform and requires 20 ul of a filtered plasma sample. The chromatographic run time is less than 15 min using a mobile phase of 15:85 v/v acetonitrile-0.18 M ammonium acetate pH 2.5. The method is good for 175 injections before replacement of the guard column. The method was linear in the range 0.5-18 ug ml(-1) (r > 0.99, n = 6) for the analytes with R.S.D. less than 10.82%. Interday and intraday variability were found to be less than 14%. The limits of detection and quantitation were 0.16 (s/n > 3) and 0.5 ug ml(-1) (s/n > 10), respectively, for each of the four benzodiazepines. (C) 2003 Elsevier B.V. All rights reserved.
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