Journal
FEBS LETTERS
Volume 555, Issue 2, Pages 192-198Publisher
WILEY
DOI: 10.1016/S0014-5793(03)01199-2
Keywords
1-Cys peroxiredoxin; 1-Cys peroxiredoxin related intronless gene 1; pseudogene; knock-out mouse
Funding
- NHLBI NIH HHS [R-01-HL65543, P-01-HL19737] Funding Source: Medline
- NIEHS NIH HHS [P-30-ES06639] Funding Source: Medline
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1-Cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine, is a unique antioxidant enzyme. We have generated mice in which the 1-cysPrx gene has been inactivated; they are viable and fertile. Mice have a highly related intronless gene (1-cysPrx-P1, GenBank accession number AF085220) with the same length of open reading frame (224 aa) as 1-cysPrx but located on a different chromosome. Since the product of this gene possibly could mimic 1-cysPrx function, we compared expression of 1-cysPrx and 1-cysPrx-P1 in mouse tissues by real-time polymerase chain reaction and Western blot. 1-cysPrx mRNA and protein were expressed in all mouse tissues that were examined with the highest expression level in lung. 1-cysPrx-P1 mRNA was expressed only in testis. In the 1-cysPrx 'knock-out' mouse, 1-cysPrx-P1 mRNA expression level was similar to the wild type but protein expression was not detected. Thus, mouse 1-cysPrx-P1 is an mRNA-expressed pseudogene that does not result in detectable protein in vivo. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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