Journal
NEUROSCIENCE LETTERS
Volume 352, Issue 2, Pages 121-124Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2003.08.042
Keywords
patch clamp; slice; dorsal horn; fluorescence; synapses; substance P; nociceptors
Categories
Funding
- NINDS NIH HHS [NS40428, NS29797] Funding Source: Medline
Ask authors/readers for more resources
Dorsal horn neurons expressing receptor for substance P (SP), the neurokinin 1 (NK1) receptor, play an important role in transmission and processing of nociceptive stimuli. To identify and study these neurons in the rat spinal cord slice preparation, we used fluorescence-conjugated SP to label NK1 receptor-expressing neurons. Labeled neurons in lamina I and III/IV were patch clamped and the vanilloid receptor 1 (TRPV1) agonist, capsaicin, was applied to evoke glutamate release from central terminals of peripheral nociceptors. Capsaicin induced an increase in the frequency of miniature excitatory postsynaptic currents in 73% of lamina I and 43% of lamina III/IV neurons expressing NK1-receptor indicating that these neurons receive direct input from capsaicin and heat sensitive nociceptors. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available