Journal
ACTA MATERIALIA
Volume 51, Issue 20, Pages 6189-6198Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S1359-6454(03)00438-5
Keywords
textural properties; polymeric resins; egg white proteins separation; methacrylate/dimethacrylate aminated supports
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Amino-functionalization of particulate macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate), obtained by bulk polymerization of a novel mixture of monomers, was carried out by reaction with concentrated ammonia, diethylamine (DEA) and the mixture DEA/THF (1: 1). The influence of the amination conditions on the polymer mechanical and thermal stability, porous structure and degree of amination was analyzed; it was also found a narrow correlation between these properties and the chromatographic behaviour of the supports. The polymers functionalized with ammonia and DEA/THF were used for hen egg white proteins separation in column packed experiments; both resins carried out the separation of a mixture of lysozyme, ovalbumin and trypsin inhibitor. The DEA/THF functionalized resin showed better performance for egg white protein separation and very good retention capacity (38 mg ovalbumin/ml resin). (C) 2003 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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