Journal
DEVELOPMENTAL BIOLOGY
Volume 264, Issue 2, Pages 495-505Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2003.08.022
Keywords
hydra; head differentiation; dsRNAi; Src; STK; protein tyrosine kinases; pattern formation
Categories
Ask authors/readers for more resources
STK, the Src tyrosine kinase homologous of the fresh water polyp hydra, is a key component of the signal transduction system for cell differentiation in this organism. Its activity is strongly increased 6 h after decapitation, and the inhibition of its activity with PP2/AG1879 prevents head development. We generated STK- polyps by using double-stranded RNA interference; STK activity of those polyps is blocked through time. STK RNAi silenced animals could not regenerate the head, but the foot, and could not reproduce asexually. The silencing of STK causes the development of ectopic heads in decapitated polyps in the first third of their body. Some head-specific genes, like Ks1, HyTcf, and Hybra1, seem to be regulated by the signaling pathway mediated by STK because their expression is modified in the STK- polyps. These findings support an important function for STK in the initial commitment of cells to develop head structures in hydra. (C) 2003 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available