Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 312, Issue 3, Pages 741-746Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2003.10.185
Keywords
SM22 alpha; smooth muscle; zebrafish; whole-mount in situ hybridization; antisense morpholino oligonucleotide; vascular; gut
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SN22alpha is a kind of 22-kDa protein which is exclusively expressed in smooth muscle containing tissues of the vertebrates. Here we report molecular cloning of a novel zebrafish SM22alpha gene. The full length of zebrafish SM22alpha cDNA is 1296 bp and it encodes a polypeptide of 201 amino acids which shares 69.2%, 69.7%, 69.2%, 67.2%, and 61.2% overall identity with human, mouse, rat, chicken, and bovine SM22alpha, respectively. Characterization of zebrafish SM22alpha genomic sequence reveals that it spans 7.7 kb and contains five exons and four introns. The expression pattern of SM22alpha in zebrafish embryonic development is studied by whole-mount in situ hybridization. Strong expression is observed in vascular, gut, swim bladder, branchial arches, and fin epidermis. Furthermore, we carry out gene knock-down by antisense morpholino oligonucleotide, which results in disappearance of yolk extension. caudal fin aberrance, and deficiency of circulation system in zebrafish embryo. Cross-section of SM22alpha-deficient embryo suggests that SM22alpha may play roles in smooth muscle cell morphology transform. (C) 2003 Elsevier Inc. All rights reserved.
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