Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 100, Issue 26, Pages 15959-15964Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2536607100
Keywords
MscS; MscL; Escherichia coli; osmoregulation
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Funding
- NIGMS NIH HHS [R01 GM37048, R37 GM037048, R01 GM037048] Funding Source: Medline
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The mechanosensitive (MS) channels MscS and MscL are essential for the survival of hypoosmotic shock by Escherichia coli cells. We demonstrate that MscS and MscL are induced by osmotic stress and by entry into stationary phase. Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing us is responsible, at least in part, for regulating production of MS channel proteins. Consistent with the model that the effect of us is direct, the MscS and MscL promoters both use RNA polymerase containing sigma(5) in vitro. Conversely, clpP or rssB mutations, which cause enhanced levels of us, show increased MS channel protein synthesis. RpoS null mutants are sensitive to hypoosmotic shock upon entry into stationary phase. These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria.
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